2020 Vol.1 Jun.  No.2

• Title: Immunomodulatory effect of a polysaccharide/polypeptide substance on a cell or mouse
• Name: Tomoko Kaneda, Asano Masumi, Chika Sakamoto
• Company: (Nihon Nihon University,Kawasaki 215-0013,Japan)
• Abstract:

  To investigate the immunoregulatory effects of certain polysaccharides/polypeptides on certain cells or mice. On the premise of adequate preparation of materials, reagents and instruments, mouse cell suspension was prepared to culture cells and observe the changes of cell morphology. Polysaccharides/polypeptides can act directly on cells or mice. The activity of certain polysaccharides/polypeptides on mice cells, the release of NO in phagocytes and the effect of polysaccharides/polypeptides on cell morphology were analyzed. The results showed that the activity of mouse cells was stronger in the concentration range of 12.5 to 50μg/mL polysaccharide/polypeptide. The concentration of 25μg/mL and 50μg/mL polysaccharide/polypeptide had strong stimulation on cells, and cells released more NO. Cell changes were more pronounced at concentrations of 25μg/mL and 50μg/mL polysaccharide/polypeptide. By analyzing the effects of polysaccharide/polypeptide on spleen lymphocyte proliferation and spleen lymphocyte secretion induction in mice, we can see that polysaccharide/polypeptide can promote spleen lymphocyte proliferation and induce spleen lymphocyte secretion in mice, indicating that polysaccharide/polypeptide have a good immune activity regulation effect on a certain cell or mouse.

  
  

• Keyword: polysaccharide/polypeptide substance; a cell or mouse; immune activity; regulation
• DOI: 10.12250/jpcarme2020010288
• Citation form: Tomoko Kaneda, Asano Masumi, Chika Sakamoto.Immunomodulatory effect of a polysaccharide/polypeptide substance on a cell or mouse.2020,Vol.1,10-15.

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